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为探讨弱光处理对转番茄Solanum lycopersicon L.GGPS2基因烟草的类胡萝卜素、叶绿素合成及耐弱光性的影响,将Sla GGPS2基因和绿色荧光蛋白报告基因(GFP)经农杆菌介导转入烟草Nicotiana tabacum L.cv.Wisconsin 38。PCR检测证明抗卡那霉素烟草含有npt II、Sla GGPS2基因,且无农杆菌污染;荧光检测发现,抗卡那霉素烟草的根尖呈现特有的荧光,由此说明获得了整合Sla GGPS2和GFP等外源基因的转基因烟草。弱光处理后,发现转Sla GGPS2基因烟草的类胡萝卜素含量、叶绿素总量、光合速率、单位叶面积重、总干重、根冠干重比均比野生烟草高,达到了差异显著水平。证实Sla GGPS2基因增加了弱光下烟草的类胡萝卜素含量、叶绿素总量,增强了光合速率,促进了生物量积累及其向根部的分配,提高了烟草弱光下的耐受性,推测可用于其他作物的耐弱光性改良。
In order to investigate the effects of low light treatment on the carotenoid, chlorophyll biosynthesis and light tolerance of transgenic Solanum lycopersicon L.GGPS2 transgenic tobacco, Sla GGPS2 gene and green fluorescent protein reporter gene (GFP) were introduced into Agrobacterium tumefaciens Tobacco Nicotiana tabacum L.cv.Wisconsin 38. PCR detection showed that the anti-kanamycin-containing tobacco contained npt II and Sla GGPS2 genes, and no Agrobacterium tumefaciens contamination. Fluorescence detection showed that the root tips of kanamycin-resistant tobacco showed unique fluorescence, thus indicating that the integrated GGPS2 and GFP and other foreign genes of transgenic tobacco. After low light treatment, it was found that the content of carotenoids, chlorophyll, photosynthetic rate, leaf area per unit area, total dry weight and dry weight per head of Sla GGPS2 transgenic tobacco were higher than that of wild tobacco, reaching the significant difference. It was confirmed that Sla GGPS2 gene increased the carotenoid content and total chlorophyll of tobacco under low light, increased the photosynthetic rate, promoted the accumulation of biomass and its distribution to the root, and increased the tolerance of tobacco under weak light. Weak light resistance to other crops.