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为探讨淹水条件下NO3-对根系抗氧化酶活性和活性氧(ROS)含量的影响,以美早/Colt甜樱桃(Prunus pseudo-cerasusLindl.)为试验材料,研究了NO3-在淹水过程中对甜樱桃根系抗氧化酶活性和活性氧含量的变化。结果表明,淹水过程中甜樱桃根系超氧化物岐化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和过氧化氢(H2O2)、超氧阴离子(O2.-)、丙二醛(MDA)含量变化皆呈先升后降趋势。加入NO3-,甜樱桃根系SOD、POD和CAT活性亦呈先升后降趋势,但均高于对照;而H2O2、O2.-和MDA含量低于对照。因此,淹水条件下,加入NO3-可以提高抗氧化酶活性、降低活性氧含量,从而减轻甜樱桃根系因淹水造成的伤害。
In order to investigate the effects of NO3- on root antioxidant enzyme activity and reactive oxygen species (ROS) content under flooding conditions, Prunus pseudo-cerasus Lindl. Was used as experimental material to study the effect of NO3- on flooding process Changes in Antioxidant Enzyme Activities and Active Oxygen Content in Roots of Sweet Cherry. The results showed that the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and hydrogen peroxide (H2O2) and superoxide anion (O2) -), MDA content increased first and then decreased. Adding NO3-, the activities of SOD, POD and CAT in roots of sweet cherry also increased at first and then decreased, but both were higher than those in the control; however, the contents of H2O2, O2.- and MDA were lower than those in the control. Therefore, under flooding conditions, the addition of NO3- can increase the antioxidant enzyme activity and reduce the reactive oxygen species content, thereby reducing the damage caused by flooding of sweet cherry root.