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目的建立HPLC-MS/MS法测定人血浆中雷喏嗪的质量浓度。方法以伏立康唑为内标,采用乙腈-体积分数0.1%甲酸水溶液(90∶10)为流动相,以Agilent-ZORBAX-C18柱(150 mm×2.1 mm,5.0μm)色谱柱为分析柱,通过电喷雾离子源(ESI),以正离子多反应监测(MRM)方式进行检测。雷喏嗪和伏立康唑用于定量分析的离子对分别为[M+H]+m/z428.5→m/z279.3和[M+H]+m/z350.2→m/z281.3。结果雷喏嗪线性范围为20~5 000 ng·mL-1,定量下限为20 ng·mL-1。日内和日间的RSD均小于15%,平均回收率大于75%。结论该方法专属性强,样品处理方便,灵敏度高,适用于雷喏嗪的临床药动学研究。
OBJECTIVE To establish a HPLC-MS / MS method for the determination of thiorazine in human plasma. Methods Voriconazole was used as an internal standard. The column was analyzed by Agilent-ZORBAX-C18 column (150 mm × 2.1 mm, 5.0 μm) with acetonitrile-0.1% formic acid as mobile phase (90:10) Spray ion source (ESI), detected by positive ion multiple reaction monitoring (MRM). The ion pairs used for quantitative analysis of thripsine and voriconazole were [M + H] + m / z 428.5 → m / z 279.3 and [M + H] + m / z 350.2 → m / z 281.3, respectively. Results The linear range of thunderrazine was 20-5 000 ng · mL-1, and the lower limit of quantification was 20 ng · mL-1. The RSDs were both less than 15% during the day and during the day, with an average recovery rate of more than 75%. Conclusion The method is highly specific, convenient for sample processing and high sensitivity. It is suitable for the clinical pharmacokinetics of thiorazine.