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低温乙醇法制备的人免疫球蛋白(IgG)静脉注射后可能引起过敏反应。原因为在制备过程中形成的聚合IgG和PKA杂质非特异性地激活了补体所致。此种聚合体可用凝胶过滤、低pH培育、活性炭或磷酸钙吸附和聚乙二醇(PEG)沉淀等办法除去。不过这样得到的单体很不稳定、不久仍会发生聚合。若加入适当物质就可防止重新聚合,从而得到稳定的完整IgG单体制剂。作者选用了尿素结合的明胶Haemaccel(H)和琥珀酰明胶Gelofusine(G)、人血白蛋白(HSA)、PEG、蔗糖、乳精、葡萄糖、果
Low-temperature ethanol prepared human immunoglobulin (IgG) may cause anaphylactic reaction after intravenous injection. This is due to the non-specific activation of complement due to the formation of polymeric IgG and PKA impurities during the preparation. Such polymers can be removed by gel filtration, low pH incubation, activated carbon or calcium phosphate adsorption and polyethylene glycol (PEG) precipitation. However, the monomer obtained in this way is very unstable, and polymerizations may still occur soon. The addition of the appropriate material prevents reaggregation, resulting in a stable, complete IgG monomer preparation. The authors selected urea-bound gelatin Haemaccel (H) and succinyl gelatin Gelofusine (G), human serum albumin (HSA), PEG, sucrose, lactulose, glucose,