二相滴定法是从1960年开始发展起来的一种新的半微量容量测定法。二相滴定最早系采用月桂基硫酸钠为滴定剂,因此,曾称为月桂基硫酸钠滴定法。近年来,随着分析技术的迅速发展,所用滴定剂及测定方法也有了新的进展。滴定原理及测定方法一、滴定原理有机碱类化合物可与月桂基硫酸钠、鲸蜡基硫酸钠、十六烷基硬脂酰硫酸钠或二辛基磺基丁二酸钠(磺基丁二酸钠二辛酯)等阴离子表面活性剂反应生成络合物,反应产物溶于有机溶剂中,而反应试剂如月桂基硫酸钠等也可与碱性染料反应。因此,可在水与有机溶剂的二相中用月桂基硫酸钠等为滴定剂,以碱性染料为指示剂,滴定有机碱。如以月桂基硫酸钠为滴定剂测定脂肪族季铵类化合物为例,滴定反应如下所 Two-phase titration is a new semi-micro-volumetric assay developed from 1960. The first two-phase titration with sodium lauryl sulfate as a titrant, therefore, has been called sodium lauryl sulfate titration. In recent years, with the rapid development of analytical techniques, there have been new advances in titrant and assay methods. Principle and determination of titration First, the principle of titration Organic base compounds with sodium lauryl sulfate, sodium cetyl sulfate, cetyl sodium stearyl sulfate or dioctyl sodium sulfosuccinate Sodium dioctate) and other anionic surfactants react to form a complex, the reaction product is dissolved in an organic solvent, and a reaction reagent such as sodium lauryl sulfate and the like may also react with a basic dye. Therefore, in the two phases of water and organic solvent with sodium lauryl sulfate as a titrant, with a basic dye as an indicator, titration of the organic base. Such as sodium lauryl sulfate titration of aliphatic quaternary ammonium compounds, for example, the titration reaction as follows