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目的 研究柴芩感冒药对呼吸道合胞病毒 (RSV)的体外抑制作用。方法 微量细胞病变抑制法。从药物的最大无毒量 (TD0 )开始 ,作连续 2倍系列稀释 6个浓度 ,每一浓度均做 4孔 ,分别加入已感染RSV的单层HeLa细胞中 ,整个实验重复 3次。按Reed Muench法计算产生 5 0 %病变抑制的药物浓度 ,即药物的半数有效量 (IC50 ) ,并计算治疗指数 (TI)。结果 柴芩感冒药的IC50 为 (31± 7 0 7)mg·L-1,TI值为 2 8 88± 4 0 5 ;中药对照金莲清热颗粒和西药对照利巴韦林注射液的IC50 和TI值分别为 (36± 5 6 6 )mg·L-1、18± 2 8和 (2 3 43± 5 0 9)mg·L-1、93± 10 7。结论 柴芩感冒药的抗RSV效果略好于金莲 ,但较利巴韦林的药效差。
Objective To study the inhibitory effect of Chaiqin cold medicine on respiratory syncytial virus (RSV) in vitro. Methods Minimal cytopathic inhibition. From the drug’s maximum non-toxic dose (TD0), serial 2-fold serial dilutions were performed at 6 concentrations. Each well was treated with 4 wells and added to monolayer HeLa cells infected with RSV. The entire experiment was repeated 3 times. The Reed Muench method was used to calculate the drug concentration that produced 50% lesion inhibition, ie, the half effective dose (IC50) of the drug, and calculate the therapeutic index (TI). RESULTS: The IC50 of Chaiqin cold medicine was (31± 7 0 7) mg·L-1, TI value was 2 8 88± 4 0 5 ; IC50 and TI of Chinese medicine control Jinlian Qingre Granules and western medicine control Ribavirin injection. The values were (36 ± 5 6 6) mg·L-1, 18 ± 2 8 and (2 3 43 ± 5 0) mg·L-1, 93 ± 10 7 respectively. Conclusion The anti-RSV effect of Chaiqin cold medicine is slightly better than that of Jinlian, but it is less effective than ribavirin.