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拟从细胞凋亡角度出发，探讨能够诱导鼻咽癌上皮细胞凋亡的因素，为有效地防治该恶性肿瘤提供新的思路。方法：光镜下观察细胞的形态变化；流式细胞仪检测细胞周期变化及亚二倍体比例；ＤＮＡ凝胶电泳及二苯胺法测定ＤＮＡ片段化程度。结果：一定浓度的拓朴异构酶Ⅰ（Ｔｏｐｏｉｓｏ－ｍｅｒａｓｅⅠ，ＴＯＰＯⅠ）抑制剂喜树碱（Ｃａｍｐｔｏｔｈｅｃｉｎ，ＣＰＴ）体外作用于人低分化鼻咽癌上皮细胞系ＣＮＥ－２Ｚ细胞一定时间后，镜下可见细胞体积缩小，染色质凝聚，胞膜突起及凋亡小体形成等形态变化。２～１０μｍｏｌ／Ｌ的ＣＰＴ分别作用１２小时后作流式细胞仪检测，亚二倍体比例均为３０％左右；各浓度分别作用２４小时，亚二倍体比例均为５０％左右；同时细胞周期有明显的变化，主要表现为Ｇ２／Ｍ期细胞减少。ＤＮＡ琼脂糖凝胶电泳呈典型的梯形带。结论：拓朴异构酶Ⅰ抑制剂ＣＰＴ对人低分化鼻咽癌上皮细胞具有明显的凋亡诱导作用。
It is intended to explore the factors that can induce the apoptosis of nasopharyngeal epithelial cells from the perspective of apoptosis and provide a new idea for the effective prevention and treatment of this malignant tumor. Methods: Morphological changes of cells were observed under light microscope. Cell cycle and sub-diploid ratio were detected by flow cytometry. DNA fragmentation was detected by DNA gel electrophoresis and diphenylamine method. RESULTS: After a certain concentration of Topoiso-meraseⅠ (CPO) inhibitor camptothecin (CPT) was applied to human poorly differentiated nasopharyngeal epithelial cell line CNE-2Z cells in vitro for a certain period of time, Volume reduction, chromatin condensation, membrane protrusion and apoptotic body formation and other morphological changes. 2 ~ 10μmol / L CPT were detected by flow cytometry 12 hours later, the sub-diploid ratio was about 30%; each concentration for 24 hours, sub-diploid ratio was about 50%; while cells Significant changes in the cycle, mainly as G2 / M phase cells decreased. DNA agarose gel electrophoresis showed a typical trapezoidal band. CONCLUSION: CPT, a topoisomerase I inhibitor, has obvious apoptosis-inducing effect on human poorly differentiated nasopharyngeal epithelial cells.