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目的:比较川芎-天麻药对不同提取物对血瘀证偏头痛大鼠的药效学差异。方法:雄性SD大鼠随机分为9组,即为正常对照组、模型对照组、阳性药盐酸氟桂利嗪(西比灵)组、川芎-天麻(4∶1,)水提物组、川芎-天麻(1∶1)水提物组、川芎-天麻(1∶4)水提物组、川芎-天麻(4∶1)醇提物组、川芎-天麻(1∶1)醇提物组、川芎-天麻(1∶4)醇提物组。大鼠冰水浴后皮下注射盐酸肾上腺素(0.8 mg/kg)和硝酸甘油(10 mg/kg)复制血瘀证偏头痛模型,比色法测定大鼠血清一氧化氮合酶(NOS)活力与一氧化氮(NO)水平,Elisa法测定血清降钙素基因相关肽(CGRP)、内皮素(ET)、血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)水平并计算NO/ET。结果:模型组血清CGRP水平、NOS活力、NO、TXB2含量及NO/ET比值升高,6-keto-PGF1α含量降低,与正常组比较差异有显著性(P<0.05);各比例提取物组间比较,各比例组醇提物NO水平及NO/ET比值均低于同比例水提物(P<0.05),6-Keto-PGF1α(4∶1组)水平高于同比例水提物(P<0.05)。结论:川芎-天麻不同提取物对血瘀型偏头痛均有一定疗效,不同提取物组间疗效有差异,综合多项指标考虑,醇提物疗效优于水提物。
OBJECTIVE: To compare the pharmacodynamic effects of Chuanxiong-Tianmai on different extracts in migraine rats with blood stasis syndrome. Methods: Male Sprague - Dawley rats were randomly divided into 9 groups: normal control group, model control group, flunarizine hydrochloride group, Chuanxiong - Gastrodia elata Blume (4:1) Chuanxiong - Tianma (1: 1) water extract group, Chuanxiong - Tianma (1: 4) water extract group, Chuanxiong - Gastrodia elata Blume (4: 1) alcohol extract group, Group, Chuanxiong - Gastrodia (1: 4) alcohol extract group. Rat models of migraine with blood stasis were subcutaneously injected with epinephrine hydrochloride (0.8 mg / kg) and nitroglycerin (10 mg / kg) after ice-water bath, and serum levels of nitric oxide synthase (NOS) The levels of serum CGRP, ET, TXB2 and 6-keto-PGF1α were measured by ELISA with Elisa method. And calculate NO / ET. Results: The levels of serum CGRP, NOS, NO, TXB2 and the ratio of NO / ET increased and the content of 6-keto-PGF1α decreased in the model group compared with the normal group (P <0.05) Compared with the same proportion of water extract (P <0.05), the levels of NO and ET in each ratio group were higher than those in the same proportion of water extract (4:1) P <0.05). Conclusion: The different extracts of Rhizoma Chuanxiong - Gastrodia elata have a certain effect on migraine with blood stasis syndrome. The curative effects of different extract groups are different. Considering multiple indicators, the effect of alcohol extract is better than that of water extract.