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建立了QuEChERS-气相色谱-串联质谱(GC-MS/MS)同时测定茶青中28种农药残留的分析方法。样品采用乙腈匀浆提取,经75 mg PSA、25 mg C18、4 mg GCB净化,GC-MS/MS多反应离子监测模式下检测,外标法定量。结果表明:在0.020~0.40 mg/L范围内,28种农药的质量浓度与对应的峰面积间呈良好的线性关系,决定系数均大于0.991。在0.10和0.50 mg/kg两个添加水平下,28种农药的平均回收率在77%~106%之间,相对标准偏差(RSD)在1.4%~8.0%(n=6)之间。方法的检出限(LOD)为5.0~20μg/kg,定量限(LOQ)为10~60μg/kg。该方法简单、快速、准确、灵敏度高,可用于茶青中多种农药残留的同时检测分析。
A method for the simultaneous determination of 28 pesticide residues in Chaqing by GC-MS / MS was established. Samples were extracted with acetonitrile homogenate, purified by 75 mg PSA, 25 mg C18, 4 mg GCB, GC-MS / MS multiple reaction ion monitoring mode, and quantified by external standard method. The results showed that there was a good linear relationship between the mass concentration of 28 pesticides and the corresponding peak area in the range of 0.020-0.40 mg / L, and the determination coefficients were all greater than 0.991. The average recoveries of 28 pesticides ranged from 77% to 106% with relative standard deviations (RSDs) between 1.4% and 8.0% (n = 6) at the two addition levels of 0.10 and 0.50 mg / kg. The limit of detection (LOD) was 5.0 ~ 20μg / kg and the limit of quantification (LOQ) was 10 ~ 60μg / kg. The method is simple, rapid, accurate and sensitive and can be used for the simultaneous detection and analysis of various pesticide residues in Chaqing.