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本文采用体外原代培养技术,详细观察和描述了大鼠胚胎下丘脑神经元在体外的形态发育过程,在国内首次建立了稳定的胚胎下丘脑神经元培养模型。结果表明:细胞培养4h,大部分细胞已贴壁;12h,部分细胞开始向外伸出小的突起;24h,部分细胞成为具有两个突起的神经元;3天时,神经元胞体增大,部分细胞为具有3个突起的神经元;1周后,神经细胞突起互相连接成网,细胞突起随培养时间延长而增多;第4周时,细胞开始减少,但仍可见较大的、具有多个突起的神经元。本文还对细胞培养过程中胚胎胎龄的选择、细胞分离的方法等问题进行了讨论。
In this paper, the in vitro primary culture technique was used to observe and describe in vitro morphological development of hypothalamus neurons in rat embryos. The stable embryonic hypothalamic neuron culture model was established for the first time in China. The results showed that most of the cells were adherent after cultured for 4h. At 12h, some of the cells started to protrude outwards. At 24h, some cells became neurons with two protuberances. At 3 days, the number of neurons was increased, The cells were neurons with three protuberances. After one week, the neurites protruded and interconnected into a network, and the protuberances of neurons increased with the prolongation of culture time. At the fourth week, the cells began to decrease, but the larger ones with multiple Protuberant neurons. This article also discusses the selection of fetal gestational age during cell culture and cell separation methods.