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目的:在细胞水平,检测“天胰素”降糖的作用途径和对葡萄糖的转化效果。方法:采用胰岛细胞β-TC-6,在正常培养液中添加0或25m M葡萄糖培养细胞,并分别给予一定浓度的“天胰素”提取液(0,100,200,400mg/L)处理,检测药物对细胞增殖影响;高糖环境中,检测细胞的线粒体功能变化及药物对线粒体的作用;检测细胞在药物处理后对葡萄糖的转化摄入。结果:(1)“天胰素”0,50,100,200和400mg/L共培养细胞,不影响细胞增殖。(2)与正常培养相比,高糖培养细胞的线粒体呼吸链功能受损,基础OCR和ATP产能OCR分别下降34.00%和28.45%。添加“天胰素”100,200和400 mg/L后,细胞线粒体呼吸链功能增强,达到正常细胞的线粒体OCR水平,基础OCR分别为:57.52±0.95,49.22±1.59和49.91±1.14(p Moles/min),ATP相关OCR分别为:55.69±0.74,50.81±1.17和48.41±1.01(p Moles/min)。(3)药物刺激下细胞摄取葡萄糖的能力显著增高:加药(100,200和400 mg/L)处理后,细胞对葡萄糖转化摄入量分别增长了25.08,25.33和28.5倍。结论:“天胰素”可增强细胞线粒体功能,促进细胞对外环境葡萄糖的摄入,提示“天胰素”有显著降糖作用。
OBJECTIVE: At the cellular level, the mechanism of hypoglycemic action and the conversion of glucose to “pachymanin” are tested. Methods: β-TC-6 islet cells were cultured in normal or dextrose medium supplemented with 0 or 25 μm glucose, and treated with certain concentrations of “Pancreatin” (0, 100, 200 and 400 mg / L) On cell proliferation; high glucose environment, the detection of cell mitochondrial function changes and the role of drugs on mitochondria; detection of cells in the drug treatment of glucose uptake. Results: (1) “Tiansuogen” 0,50,100,200 and 400mg / L co-cultured cells, does not affect cell proliferation. (2) The mitochondrial respiratory chain function was impaired in high glucose cultured cells compared with normal culture, OCR of basic OCR and ATP decreased by 34.00% and 28.45%, respectively. After adding 100, 200 and 400 mg / L of “Psoriasis”, the mitochondrial respiratory chain function of the cells was enhanced and reached the normal mitochondrial OCR levels of 57.52 ± 0.95, 49.22 ± 1.59 and 49.91 ± 1.14 (p Moles / min) and ATP-related OCRs were 55.69 ± 0.74, 50.81 ± 1.17 and 48.41 ± 1.01 (p Moles / min), respectively. (3) The ability of cells to uptake glucose was significantly increased under drug-stimulated treatment: the cell uptake of glucose increased by 25.08, 25.33 and 28.5 folds after dosing (100, 200 and 400 mg / L) respectively. Conclusion: “Tiansuogen” can enhance mitochondrial function of cells and promote the uptake of glucose into the external environment, suggesting that “Tiansuogen” has a significant hypoglycemic effect.