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作者制备了在3′端连接6个生物素分子的表皮生长因子受体(EGF-R)特异性负链寡核苷酸探针,由24个碱基组成(5′GGA′GCG′CTG′CCC′CGG′CCG′TCC′CGG3′),它与EGF-R mRNA互补。用有30个脱氧胸苷酸链的寡核苷酸探针证明mRNA的完整性并与目的EGF-R基因100％同源。用原位杂交(ISH)及抗生物素碱性磷酸酶显色系统,检测福尔马
The authors prepared epidermal growth factor receptor (EGF-R)-specific negative-strand oligonucleotide probes that bind 6 biotin molecules at the 3′ end and consist of 24 bases (5′GGA′GCG′CTG′. CCC′CGG′CCG′TCC′CGG3′), which is complementary to EGF-R mRNA. Oligonucleotide probes with 30 deoxythymidylate chains demonstrated the integrity of the mRNA and were 100% homologous to the EGF-R gene of interest. Detection of Formosa using in situ hybridization (ISH) and an anti-biotin alkaline phosphatase color system