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目的:探讨米非司酮对人胚胎细胞JAR与人子宫内膜细胞RL95-2之间黏附的影响及其调控的分子机制。方法:采用黏附实验分别观察RU486以及岩藻糖基转移酶IV(FUT4)对JAR细胞与RL95-2细胞之间黏附的影响。采用RT-PCR以及Western blotting检测米非司酮对RL95-2细胞以及流产妇女子宫内膜中FUT4表达的调控。结果:①米非司酮抑制JAR细胞与RL95-2单层细胞之间的黏附;②米非司酮抑制RL95-2细胞以及流产妇女子宫内膜中FUT4基因和蛋白的表达;③FUT4质粒转染RL95-2细胞后,调节JAR细胞与RL95-2单层细胞之间的黏附。结论:米非司酮通过调节FUT4的表达抑制胚胎体外黏附。
Objective: To investigate the effect of mifepristone on the adhesion between JAR and human endometrial cell line RL95-2 and its molecular mechanism. Methods: Adhesion experiments were performed to observe the effect of RU486 and fucosyltransferase IV (FUT4) on the adhesion between JAR cells and RL95-2 cells. RT-PCR and Western blotting were used to detect the effect of mifepristone on the expression of FUT4 in RL95-2 cells and endometrium of aborted women. Results: (1) Mifepristone inhibited the adhesion between JAR cells and RL95-2 monolayer cells; (2) Mifepristone inhibited the expression of FUT4 gene and protein in RL95-2 cells and endometrium of aborted women; (3) FUT4 plasmid transfection RL95-2 cells, regulate the adhesion between JAR cells and RL95-2 monolayers. Conclusion: Mifepristone inhibits the in vitro adhesion of embryos by regulating the expression of FUT4.