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B3超家族是一类含有B3功能域(与DNA结合的高度保守结构域)的转录因子,在植物生长发育过程中起重要作用。本研究采用生物信息学的方法,利用Pfam中的B3保守结构域序列检索番茄(Solanum lycopersicum L.)蛋白序列,确定了97个B3超家族基因。对番茄B3超家族成员进行了系统进化树分析、染色体定位、结构域分析、组织表达和诱导表达分析等。番茄B3超家族分为LAV、ARF、RAV和REM 4个亚家族,每个亚家族中的数量分别为4、22、9和62个,且在进化树中形成明显不同的分支,每个亚家族都进行了系统进化和结构域分析;番茄12条染色体都含有B3超家族基因;11个成员的表达模式表明,B3超家族同一亚家族成员也具有不同的时空表达模式;在干旱、盐和高温胁迫处理下,部分成员响应强烈并且响应不同的外界信号;而对于ABA处理响应非常弱。本研究将为B3基因超家族成员的生物学功能研究提供参考。
The B3 superfamily is a class of transcription factors that contain the B3 domain (a highly conserved domain that binds to DNA) and plays an important role in plant growth and development. In this study, we used the method of bioinformatics to search the protein sequence of tomato (Solanum lycopersicum L.) using the B3 conserved domain of Pfam, and identified 97 B3 superfamily genes. Phylogenetic tree analysis, chromosome mapping, domain analysis, tissue expression and induced expression analysis of tomato B3 superfamily members were conducted. The tomato B3 superfamily is divided into four subfamilies, LAV, ARF, RAV and REM. The number of subfamilies in each subfamily is 4, 22, 9 and 62, respectively, and distinctly different branches are formed in the evolutionary tree. All 12 families of tomato chromosomes contain B3 superfamily genes. The expression patterns of 11 members indicate that the members of the same subfamily of B3 superfamily also have different temporal and spatial expression patterns. In drought, salt and Under high temperature stress, some members responded strongly and responded to different external signals, while the response to ABA treatment was very weak. This study will provide reference for the biological function of B3 gene superfamily members.