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目的:探讨农吉利碱对乳腺癌细胞MDA-MB-231增殖与凋亡的影响。方法:体外培养MDA-MB-231细胞,给予不同药物浓度(0、10、20、40μmol/L)农吉利碱处理MDA-MB-231细胞24、48、72h。四甲基偶氮唑盐比色(MTT)法检测对MDA-MB-231细胞活力的影响,光镜和吖啶橙染色分析细胞形态,流式细胞术检测农吉利碱处理细胞72h后的凋亡率和细胞内活性氧水平,免疫印迹法(Western blot)检测B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、激活型半胱氨酸蛋白酶-3(Cleaved-Caspase-3)蛋白表达。结果:农吉利碱10、20、40μmol/L对MDA-MB-231细胞具有明显抑制作用,光镜、吖啶橙染色法可见细胞出现凋亡特征性改变,农吉利碱可诱导细胞凋亡,抑制Bcl-2蛋白表达,促进Bax和Cleaved-Caspase-3的蛋白表达,并与农吉利碱浓度呈依赖性。结论:农吉利碱抑制乳腺癌细胞MDA-MB-231增殖,并促进其凋亡,与Bax和Cleaved-Caspase-3的表达增加和Bcl-2降低有关。
Objective: To investigate the effect of Nongli Li base on the proliferation and apoptosis of breast cancer cell line MDA-MB-231. Methods: MDA-MB-231 cells were cultured in vitro, and treated with nonglitizine at different concentrations (0, 10, 20 and 40 μmol / L) for 24, 48, and 72 hours. The effect of MTT assay on the viability of MDA-MB-231 cells was observed. The morphology of MDA-MB-231 cells was observed by light microscopy and acridine orange staining. Flow cytometry (Bcl-2), Bax, Bcl-2, Cleaved-3 and Bcl-2 were measured by Western blot. -Caspase-3) protein expression. Results: 10, 20, 40μmol / L Nongwei Li significantly inhibited the proliferation of MDA-MB-231 cells. Apoptosis was observed by light microscopy and acridine orange staining. Nongji Li alkali could induce apoptosis, Inhibited the expression of Bcl-2 protein, promoted the protein expression of Bax and Cleaved-Caspase-3, and was dependent on the concentration of Nongiteli. CONCLUSION: Nonglishith can inhibit the proliferation and promote the apoptosis of breast cancer cell MDA-MB-231, which is related to the increase of Bax and Cleaved-Caspase-3 expression and the decrease of Bcl-2.