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目的:研究运动训练大鼠长期停训后海马神经元特异性烯醇化酶的表达变化。方法:参照Bedford跑台训练方案分别建立雄性SD大鼠有氧训练与疲劳训练模型,停训28周后,应用免疫组织化学技术探讨长期停训对大鼠脑海马组织神经元特异性烯醇化酶(Neuron-Specific enloase,NSE)表达的影响。结果:有氧训练与疲劳训练均抑制大鼠体重的生长,且疲劳训练的抑制作用尤为明显。恢复28周后,体重均不同程度的增加,且疲劳训练大鼠体重增加幅度最大。停训恢复28周后,疲劳训练大鼠海马CA1区NSE的表达明显上调;在海马CA3区,疲劳训练与有氧训练上调NSE的表达不明显。结论:疲劳训练后停训可上调海马神经元特异性烯醇化酶的表达,这可能与疲劳训练使海马CA1区缺氧微损伤相关。
OBJECTIVE: To study the changes of neuron specific enolase in hippocampus after exercise training for a long time. Methods: According to the Bedford treadmill training program, the male SD rats aerobic training and fatigue training model were established. After 28 weeks training, the use of immunohistochemical techniques to explore long-term suspension training on rat hippocampus neuron-specific enolase (Neuron-Specific enloase, NSE) expression. Results: Both aerobic training and fatigue training inhibited the growth of body weight in rats, and the inhibitory effect of fatigue training was particularly obvious. After 28 weeks of recovery, the body weight increased to varying degrees, and fatigue training rats gained the largest weight gain. The expression of NSE in the hippocampal CA1 region of fatigue-trained rats was significantly increased after 28-week recovery training. In hippocampal CA3 region, the expression of NSE was not significantly increased by fatigue training and aerobic training. Conclusion: After training, fatigue training can increase hippocampal neuron specific enolase expression, which may be associated with fatigue training to hypoxia microinjury in hippocampal CA1 area.