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以豆科药用植物降香檀、决明、含羞草、灰毛豆和猪屎豆的成熟种子为材料,探讨含水量对其发芽率的影响,以及超低温冷冻方式对种子超低温保存的影响。结果表明,降香檀、决明、猪屎豆和灰毛豆种子发芽率均随含水量的下降而从80%左右降至20%以下,而含羞草种子含水量低于10%时,其发芽率仍在75%以上。经超低温冷冻后,五种豆科药用植物种子发芽率较对照组均有显著差异;适宜的含水量下,种子经过超低温冷冻后其发芽率与对照组差异不显著,甚至高于对照组。三种冷冻方法中,玻璃化冷冻法更适合降香檀种子的超低温保存,缓慢冷冻法更适合猪屎豆种子的超低温保存,快速冷冻法适合于决明种子、灰毛豆种子和含羞草种子的超低温保存。由此可知,液氮超低温冷冻法保存降香檀等五种豆科药用植物种子是可行的。
The effects of water content on the germination rate and the effects of ultra-low temperature freezing methods on cryopreservation of seed were studied using the mature seeds of Medicago sativa, Cassia obtusifolia, Mimosa, Grifola frondosa and Crotalaria sativus as materials. The results showed that the germination rate of Desmodium Dalbergiae, Cassia thompsonii, Crotalaria delavayi and Siberian soybean germination decreased from about 80% to below 20% with the decrease of water content, while the germination rate of Mimosaceae seed with water content below 10% Rate is still above 75%. After cryopreservation, the seed germination rate of five leguminous medicinal plants were significantly different from that of the control group. Under suitable water content, the germination rate of the seeds after cryopreservation was not significantly different from that of the control group. Among the three freezing methods, the vitrification method is more suitable for the cryopreservation of the fragrant sandalwood seeds, the slow freezing method is more suitable for the cryogenic preservation of the crotalaria seeds, the rapid freezing method is suitable for the seeds of the cassia seed, the gray soybean seed and the mimosa seed Cryogenic preservation. It can be seen that cryopreservation of liquid nitrogen to save fragrant sand and other legumes five legumes is feasible.