论文部分内容阅读
将拟南芥AtCBF1、AtCBF2和AtCBF3基因簇完整地导入番茄基因组,以期修正番茄CBF冷应答系统的遗传缺陷,提高番茄的耐寒性.结果显示,转基因番茄植株中外源AtCBF1、AtCBF2和AtCBF3基因能被4℃低温诱导表达,6h后相对表达量均提高大约200倍;低温胁迫下,转基因番茄的细胞膜透性和丙二醛(MDA)含量的增加量显著低于野生型对照,而超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性增强幅度显著高于野生型材料,从而使转基因番茄的耐寒性得到显著提高.
The AtCBF1, AtCBF2 and AtCBF3 Arabidopsis thaliana genomes were completely introduced into the genome of tomato so as to correct the genetic defects in the cold response system of tomato CBF and improve the cold tolerance of tomato. The results showed that the exogenous AtCBF1, AtCBF2 and AtCBF3 genes in transgenic tomato plants were 4 ℃ low temperature induced expression, after 6h relative expression increased about 200 times; under low temperature stress, the transgenic tomato membrane permeability and MDA content was significantly lower than the wild-type control, and superoxide dismutase Enzyme (SOD), peroxidase (POD) and catalase (CAT) activity increased significantly higher than the wild-type material, so that the cold tolerance of transgenic tomatoes was significantly improved.