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用 HPL C测定了过氧化物酶体膜磷脂的含量。用 Folch法提取膜脂质 ,以 μ-Porasil Si60为固定相 ,以乙腈-甲醇 -磷酸为流动相 ,采用梯度洗脱 ,紫外检测波长 2 0 5 nm。标准回收率 :心磷脂 (C)为 (1 0 3 .97± 1 2 .5 7) % ,磷脂酰肌醇 (PI)为 (88.2 3± 5 .42 ) % ,磷脂酰丝氨酸 (PS)为 (90 .3 3± 6.84) % ,磷脂酰乙醇胺 (PE)为 (84.41±1 0 .2 2 ) % ,磷脂酰胆碱 (PC)为 (89.78± 8.70 ) % ,鞘磷脂 (SM)为 (84.0 4± 1 2 .0 6) %。最小检出限分别为 :C1 2ng,PI 8ng,PS 2 3 ng,PE 4ng,PC 2 3 ng,SM 7ng。线性关系与回收率较好 ,结果令人满意。
Peroxisome membrane phospholipid content was determined by HPL C. Folch method was used to extract membrane lipids. The mobile phase consisted of μ-Porasil Si60 with acetonitrile-methanol-phosphoric acid as the mobile phase and gradient elution was used. The UV detection wavelength was 205 nm. Standard recovery was (103.97 ± 1.27.5%) for cardiolipin (88.23 ± 5.42)%, phosphatidylserine (PS) was (90.33 ± 6.84)%, phosphatidylethanolamine (PE) was 84.41 ± 102.2%, phosphatidylcholine was 89.78 ± 8.70% and sphingomyelin was ( 84.0 4 ± 1 2 .0 6)%. The minimum detection limits were: C1 2 ng, PI 8 ng, PS 2 3 ng, PE 4 ng, PC 2 3 ng, SM 7 ng. Linear relationship and recovery rate is good, the result is satisfactory.