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目的 探讨抗肿瘤坏死因子α单克隆抗体 (anti-TNFα m Ab)对急性胰腺炎所致全肠外营养大鼠肠屏障损伤的影响 .方法 SD大鼠 32只 ,采用 35 g· L- 1 牛磺胆酸钠逆行胰胆管注射建立急性胰腺炎模型后 ,随机分为对照组 (n=1 6 )及实验组 (n=1 6 ) ,对照组给予全肠外营养支持 ,实验组给予相同配方的全肠外营养支持 ,并予 anti- TNFα m Ab静脉内注射 .于建立急性胰腺炎模型后 1及 5 d处死 ,检测大鼠肠道通透性、空肠粘膜湿重、微绒毛高度和面积、双糖酶活性及髓过氧化物酶活性 .结果 治疗后两组动物间肠道通透性、空肠粘膜湿重、微绒毛高度及面积、双糖酶活性及髓过氧化物酶活性均无显著差别 .结论 anti- TNFα m Ab对急性胰腺炎全肠外营养大鼠肠屏障功能无明显影响
Objective To investigate the effect of anti-TNFα m Ab on the intestinal barrier injury in rats with parenteral nutrition induced by acute pancreatitis.Methods Thirty-two SD rats (35 g · L -1) The rats were randomly divided into control group (n = 16) and experimental group (n = 16). The control group was given parenteral nutrition support. The experimental group was given the same formula Of the total parenteral nutrition support and anti-TNFα m Ab intravenous injection in the establishment of acute pancreatitis model 1 and 5 d after sacrifice, detection of intestinal permeability, jejunal mucosal wet weight, microvilli height and area , Disaccharidase activity and myeloperoxidase activity.Results The intestinal permeability, wet weight of jejunum mucosa, microvilli height and area, disaccharidase activity and myeloperoxidase activity Significant difference.Conclusion Anti-TNFα m Ab had no significant effect on gut barrier function in rats with parenteral nutrition after acute pancreatitis