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目的探讨噬菌体裂解法在基层结核病防治机构实验中的应用价值。方法应用噬菌体裂解法、改良罗氏培养法及直接涂片法同时检测250份临床上确诊为肺结核初诊患者的痰标本。结果①250份痰标本用噬菌体裂解法、改良罗氏培养法及直接涂片法检出的阳性率分别为50.1%、43.2%、27.6%。②噬菌体裂解法的阳性检出率远高于涂片法的阳性检出率,两者的差异有统计学意义(χ2=28.23,P<0.01);噬菌体裂解法的阳性检出率高于改良罗氏培养法的阳性检出率,但两者的差异无统计学意义(χ2=2.90,P>0.05)。结论噬菌体裂解法能快速、简便地检测结核分枝杆菌,且检出率较高,可作为结核病诊断的重要方法,且无需特殊设备,在普通微生物实验室中就能开展,适合在基层结核病防治机构实验室中推广应用。
Objective To explore the value of bacteriophage lysis in the experiment of primary prevention and treatment of tuberculosis. Methods Bacteriophage lysis method, modified Roche culture method and direct smear method were used to detect sputum samples of 250 clinically diagnosed newly diagnosed tuberculosis patients. Results ① The positive rates of 250 sputum samples detected by phage lysis method, modified Roche culture method and direct smear method were 50.1%, 43.2% and 27.6% respectively. ② The positive detection rate of phage lysis method was much higher than that of smear method, the difference was statistically significant (χ2 = 28.23, P <0.01); the positive detection rate of phage lysis method was higher than that of modified Roche culture positive detection rate, but the difference between the two was not statistically significant (χ2 = 2.90, P> 0.05). Conclusion The bacteriophage lysis method can detect Mycobacterium tuberculosis quickly and easily with a high detection rate, which can be used as an important method for diagnosis of tuberculosis. It can be carried out in ordinary microbiological laboratories without special equipment and is suitable for the prevention and treatment of tuberculosis Promotion and application in institutional laboratories.