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用２４个在我国具有代表性的小麦白粉菌已知毒性菌株，对两个不同来源的小麦一黑麦６ＢＳ＿６ＲＬ易位系进行了抗病性鉴定，表明 Ｐｍ２０基因已发生抗谱分化．以这两个材料为亲本配制 ＴＡＭ１０４Ｒ×ＴＡＭ１０４Ｓ杂交组合获得 Ｆ２分离群体，对亲本和 Ｆ２分离群体进行 ＡＦＬＰ分析．亲本 ＤＮＡ经 ＰｓｔⅠ／ＴａｑⅠ双酶切消化，并接上各自的接头，然后以其接头为基础加１个或３个碱基，分别经过两次ＰＣＲ扩增，结果 １６对 ＰｓｔⅠ／ＴａｑⅠ引物组合（４个 ＰｓｔⅠ引物， ４个 ＴａｑⅠ引物）ＰＣＲ扩增出 ２２９０条带，共获得 ３个与该基因连锁的分子标记，分别位于 Ｐｍ２０基因的一端 ２．６和 １１．６ ｃＭ，另一端 ３．６ ｃＭ处，实现了对 Ｐｍ２０基因的连锁分析．
Twenty four wheat-rye translocation lines with different provenances were identified by 24 known virulent strain of wheat powdery mildew in our country, which indicated that the Pm20 gene had anti-spectrum differentiation. The TAM104R × TAM104S crosses were prepared from these two materials as parents to obtain the F2 segregation population, and the AFLP analysis was performed on the F2 population. The parental DNA was double digested with PstI / TaqI and ligated to their respective adapters, followed by addition of one or three bases to their adapters, respectively, after two PCR amplifications. As a result, 16 pairs of PstI / TaqI primer combinations 4 PstⅠprimer and 4 TaqⅠprimer) were used to amplify 2290 bands. Three molecular markers linked to this gene were obtained, which were located at 2.6 and 11.6 cM at one end of Pm20 gene and 3.6 at the other end respectively cM Department, to achieve a linkage analysis of the Pm20 gene.