本研究通过对溴乙淀染色、紫外分光光度计和高效液相色谱(HPLC)等方法的比较,找出了适用于不同情况下,微量DNA的最佳测定方法:1.紫外分光光度计,能准确测量纯净的、浓度在1μg/mL以上的DNA浓度;2.在有标准DNA的情况下,溴乙淀染色法能粗略区别1～5ngDNA;3.HPLC能够快速、准确检测0.21ng以上的DNA,此法简便、灵敏、不受杂质干扰,且能同时对多种微量DNA进行分离和定量。 In this study, the best method for determination of trace DNA suitable for different conditions was found through the comparison of bromoethyl bromide staining, ultraviolet spectrophotometer and high performance liquid chromatography (HPLC): 1. UV spectrophotometer, Can accurately measure the purity of DNA concentration of 1μg / mL or more; 2 in the case of a standard DNA, bromothiocyanate staining method can roughly distinguish 1 ~ 5ngDNA; 3.HPLC can quickly and accurately detect 0.21ng above DNA, this method is simple, sensitive, free from impurity interference, and can simultaneously separate and quantify a variety of trace DNA.