论文部分内容阅读
以小麦品种小偃81为材料,利用低能N+离子,60Coγ射线以及紫外线UV-C等为诱变源,对种子胚部进行辐照处理。研究其对小麦发芽势、发芽率、根长及苗高等生理指标的影响。以辐照后的幼苗为材料研究了低能N+注入对过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性及还原型谷胱甘肽(GSH)含量的影响。研究结果表明:N+注入后种子活力先升高后降低,苗高在5×1017 N+/cm2时显著低于对照;60Co和紫外线辐照对种子活力有较显著的影响,辐照后的根长和苗高均显著低于对照;N+束辐照后的幼苗CAT酶活随剂量的变化规律性不明显,POD和SOD酶活总体趋势随剂量的增加先升高后降低,GSH含量随剂量的增大先降低后升高。由此可知,辐照诱变可以诱导小麦一系列的生理变化。60Coγ射线和紫外线UV-C对小麦胚根的伤害较大,导致幼苗在后期大量死亡。N+注入对小麦的损伤效应较60 Co和紫外线γ射线和紫外线UV-C小,一定注量的N+注入处理可促进小麦生长。
The wheat embryo was irradiated with low energy N + ions, 60Co γ-rays and UV-C as the mutagenesis source. The effects of wheat germination potential, germination rate, root length and seedling height on physiological indices were studied. The effects of low energy N + implantation on the activities of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and glutathione (GSH) in irradiated seedlings were studied. Impact. The results showed that the seed vigor increased firstly and then decreased, the seedling height was significantly lower than that of the control at the seedling height of 5 × 10 17 N + / cm 2. The 60Co and ultraviolet radiation had a significant effect on seed vigor. The root length And seedling height were significantly lower than the control; CAT activity of seedlings with N + irradiation had no obvious change with the dose, the overall trend of POD and SOD activity first increased and then decreased with the increase of dose, GSH content with dose Increase first reduce and then increase. This shows that irradiation induced mutagenesis can induce a series of physiological changes in wheat. 60Coγ ray and ultraviolet UV-C harmed wheat radicle, leading to a large number of seedlings died in the late. The damage effect of N + implantation on wheat was less than that of 60 Co and UV-γ rays and UV-C. A certain amount of N + injection could promote wheat growth.