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将纯化的西瓜花叶病毒(Watermelon mosaic virus,WMV)制剂免疫BALB/c小鼠,用SP2/0骨髓瘤细胞与经西瓜花叶病毒免疫的BALB/c小鼠的脾细胞融合,有限稀释法克隆和间接ELISA法筛选出1株稳定分泌西瓜花叶病毒单克隆抗体的杂交瘤细胞株6C6。用间接ELISA方法对所获得的杂交瘤细胞株进行亚型鉴定为IgG1。间接ELISA方法测定腹水效价为1∶105。以单克隆抗体为包被抗体、多克隆抗体为检测抗体的TAS-ELISA试剂盒与引自ATCC的西瓜花叶病毒毒源PV-27、PV-379、PV-394、PV-511分离物均有反应,与同属的马铃薯A病毒(Potato virus A)、莴苣花叶病毒(Lettuce mosaicvirus virus)、李痘病毒(Plum pox virus)不发生交叉反应,与同属的番木瓜环斑病毒呈弱阳性反应。。
BALB / c mice were immunized with a purified formulation of Watermelon mosaic virus (WMV), spleen cells were fused with SP2 / 0 myeloma cells and BALB / c mice immunized with watermelon mosaic virus, and the limiting dilution method A hybridoma cell line 6C6 stably secreting monoclonal antibody against watermelon mosaic virus was screened out by cloning and indirect ELISA. The obtained hybridoma cell line was sub-typed as IgG1 by an indirect ELISA method. Indirect ELISA method for the determination of ascites titer 1:105. TAS-ELISA kit with polyclonal antibody as detection antibody and PV-27, PV-379, PV-394 and PV-511 isolates from the virulent sources of watermelon mosaic virus Response to the same genus Potato virus A, Lettuce mosaicvirus virus, Plum pox virus did not cross-react with the same papaya ringspot virus was weakly positive reaction . .