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本研究利用日本晴与泸恢99构建的188个重组自交系群体,根据已构建的遗传连锁图谱,采用混合线性模型方法,在高镉含量环境A和低镉含量环境B进行糙米Cd~(2+)含量的QTL定位分析。在A环境中检测到2个控制糙米Cd~(2+)含量的QTLs,分别位于第7、8染色体,贡献率分别为29.8%、8.87%;在B环境中检测到1个控制糙米Cd~(2+)含量的QTL,位于第7染色体,贡献率为7.35%;A、B两个环境下的增效基因均来自于亲本泸恢99。试验表明:镉浓度对该基因的表达有影响,糙米Cd~(2+)含量QTL基因更易在Cd~(2+)高浓度环境中表达,因此,Cd~(2+)低吸收水稻材料创制及选育应在Cd~(2+)含量较高的土壤中进行。
In this study, 188 recombinant inbred lines constructed by Nipponbare and Luhui 99 were used in this study. Based on the constructed genetic linkage map, mixed linear models were used to study the effects of Cd ~ (2) +) Content of QTL mapping analysis. Two QTLs controlling the content of Cd 2+ in brown rice were detected in A environment, which were located on chromosomes 7 and 8, respectively, with the contribution rates of 29.8% and 8.87%, respectively. In the B environment, (2+) content of QTL, located on chromosome 7, the contribution rate of 7.35%; A, B two environment synergistic genes are from the parent Lu Hui 99. (2) The results showed that cadmium concentration had an influence on the expression of this gene, and the QTL gene of brown rice Cd 2+ content was more likely to be expressed in Cd 2+ high concentration environment. Therefore, Cd 2+ low- And breeding should be carried out in soils with higher Cd2 + content.