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目的探讨茶油多种临床用药可能的药理药效基础。方法将油茶籽经两次95%的乙醇回流提取后浓缩至不含乙醇和水的提取物,取其中的茶油部分作为试验药物;用肿瘤坏死因子-α(TNF-α)处理人乳腺癌细胞系(MCF-7)细胞建立细胞炎症模型,进一步用蛋白质印迹技术观察药物处理对细胞炎症因子IκB-α表达的影响进而评价茶油是否具有抗炎活性。结果从油茶籽提取得到的茶油显著地抑制细胞中TNF-α诱导的IκB-α的降解从而抑制炎症发生。结论茶油对细胞显示良好的抗炎作用,这种特性可能是其临床应用于治疗皮肤过敏、暗疮、疥癣、小儿红屁股、以及皮肤烧烫伤等多种与炎症相关疾病的药理基础之一。
Objective To investigate the possible pharmacological efficacy of tea oil in clinical medicine. Methods Camellia seeds were extracted by refluxing twice with 95% ethanol and concentrated to extracts without ethanol and water. The tea oil fraction was used as experimental drug; and human breast cancer was treated with tumor necrosis factor-α (TNF-α). The cell line (MCF-7) cells were used to establish a cell inflammation model, and the effect of drug treatment on the expression of IκB-α was further examined by Western blotting to evaluate whether the tea oil had anti-inflammatory activity. Results The tea oil extracted from Camellia oleifera seeds significantly inhibited the degradation of IκB-α induced by TNF-α in the cells and inhibited the occurrence of inflammation. Conclusion Tea oil shows good anti-inflammation effect on cells, which may be one of the pharmacological bases of its clinical application in the treatment of skin allergies, acne, sputum, pediatric red butt, and skin burns. .