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用邻苯二甲醛 (OPA) -3_巯基丙酸 (3_MPA)柱前衍生高效液相色谱法测定了采自大亚湾近岸海域的一个长60cm的沉积物柱样W0中15种游离氨基酸 (FAA)的含量 ;结果表明 ,W0 中FAA总量在0.82～2.69mmol/kg之间 ,15种FAA以及FAA总量均随深度而以指数曲线降低 ;对氨基酸组成的分析表明 ,天冬氨酸、甘氨酸、组氨酸和苯丙氨酸的摩尔分数随深度而上升 ,苏氨酸、精氨酸、缬氨酸和异亮氨酸的摩尔分数随深度而下降 ,其他氨基酸的摩尔分数随深度无显著变化。甘氨酸、丙氨酸、天冬氨酸、谷氨酸、丝氨酸是W0中含量最丰富的氨基酸 ,其摩尔分数依次为28.1%、16.0 %、14.7 %、10.6%和7.2 %。
The determination of 15 free amino acids (FAA) in a 60 cm long sediment column W0 collected from the coastal area of the Daya Bay by using pre-column derivatization with OPA-3-mercaptopropionic acid (3_MPA) ). The results showed that the total amount of FAA in W0 ranged from 0.82 to 2.69 mmol / kg, and the total amount of 15 kinds of FAA and FAA decreased with exponential curve. The analysis of amino acid composition showed that aspartic acid, The molar fractions of glycine, histidine and phenylalanine increased with depth, and the molar fractions of threonine, arginine, valine and isoleucine decreased with depth, while those of other amino acids decreased with depth Significant changes. Glycine, alanine, aspartic acid, glutamic acid and serine are the most abundant amino acids in W0 with mole fractions of 28.1%, 16.0%, 14.7%, 10.6% and 7.2%, respectively.