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目的研究谷胱甘肽S-转移酶(GSTs)在德国小蠊不同发育阶段(若虫到成虫)与北京地区不同野外种群中的活性水平。方法分别采用分光光度法与药膜法测定德国小蠊GSTs活性与其抗性水平。结果德国小蠊GSTs比活力在若虫期随龄期增加而逐渐升高,在4周龄达到最高;随后逐渐降低,在羽化时酶活力降至最低,在成虫期略有升高。德国小蠊敏感品系成虫GSTs比活力为2.70 nmol(/min.mg),Km值与Vmax分别为2.26 mmol/L和0.33 nmol(/min.mg);9个区(县)野外种群GSTs比活力为2.76～8.69 nmol(/min.mg),Km值和Vmax分别为0.65～2.37 mmol/L和0.33～0.54 nmol(/min.mg)。与敏感品系比较,野外种群成虫GSTs比活力和Vmax均较高,Km值较低,且对2种常用杀虫剂表现出不同程度的抗性(对高效氯氰菊酯为1.50～8.37倍;对残杀威为1.04～3.34倍)。结论 GSTs活性随德国小蠊发育阶段不同(若虫到成虫)而变化,并可能参与德国小蠊野外种群的抗性形成。
Objective To study the activity of glutathione S-transferase (GSTs) in different wild populations of Blattella germanica at different developmental stages (nymphs to adults) and Beijing area. Methods The GSTs activity and resistance level of Blattella germanica were determined by spectrophotometry and membrane assay respectively. Results The specific activity of GSTs of German cockroach increased gradually with the increase of age at nymphs, reached the highest at 4 weeks of age, then decreased gradually and decreased to the lowest during emergence and slightly increased during adult stages. The specific activity of GSTs of German cockroach susceptible strains was 2.70 nmol (/min.mg), the Km value and Vmax were 2.26 mmol / L and 0.33 nmol (/min.mg), respectively. The specific activities of GSTs Was 2.76 ~ 8.69 nmol (/min.mg), Km and Vmax were 0.65 ~ 2.37 mmol / L and 0.33 ~ 0.54 nmol (/min.mg) respectively. Compared with the susceptible lines, the specific activities and Vmax of GSTs in field were higher and the Km values were lower. The resistance to 2 commonly used insecticides was 1.50 ~ 8.37 times that of cypermethrin. 1.04 to 3.34 times). Conclusions The activity of GSTs varies with different stages of development of Blattella germanica (nymphs to adults) and may be involved in the resistance formation of the wild population of Blattella germanica.