论文部分内容阅读
为了鉴定随机扩增产物的同源性以及更有效地利用DNA分子标记研究人参种质资源,我们首次将毛细管PCR扩增的RAPD反应产物进行限制性内切酶消化并有效的进行了酶切位点分析,结果证明PCR-RFLP是筛选人参特异DNA分子标记的一种简单易行的新方法,可为药用植物种质资源研究提供另一有用的工具。
In order to identify the homology of random amplified products and to use DNA molecular markers to study Ginseng germplasm resources more effectively, we first performed restriction enzyme digestion and efficient enzymatic cleavage of the RAPD reaction products amplified by capillary PCR. The point analysis showed that PCR-RFLP was a simple and easy method for screening ginseng specific DNA molecular markers, and could provide another useful tool for the research of medicinal plant germplasm resources.