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目的 P物质 (SP)是气道非胆碱能兴奋性神经的重要递质 ,是导致哮喘气道神经源性炎症产生的一种重要感觉神经肽。糖皮质激素是否有降低肺内SP的效应尚不清楚 ,为此本文研究了吸入糖皮质激素对哮喘豚鼠肺内SP的影响及其分子机制。方法 30只豚鼠用卵蛋白致敏、诱喘制成哮喘动物模型 ,随机分为哮喘组和治疗组 (每组 1 5只 ) ,另 1 5只豚鼠吸入生理盐水作为对照组。治疗组豚鼠在诱喘前 1天、诱喘当天及诱喘后 2 4h吸入丙酸倍氯米松雾化溶液。哮喘组和治疗组豚鼠均于诱喘后 2 4h取材 ,对照组同期取材 ,分别留取支气管肺泡灌洗液 (BALF)、血浆及肺组织标本。用放射免疫放法检测SP蛋白含量 ;用反转录多聚酶链式反应检测肺组织SPmRNA相对含量。结果 哮喘组豚鼠血浆、BALF及肺组织SP含量 [(1 2 2± 4 6 )pg/mL ,(90± 39) pg/mL ,(78±15 )pg/克蛋白 ]均明显高于正常对照组 [(84± 33) pg/ml,(32± 2 1 ) pg/ml,(4 2± 1 2 ) pg/ml]及激素治疗组 [(5 0±1 3) pg/mL ,(4 7± 2 0 )pg/mL ,(4 0± 1 3) pg/克蛋白 ],其差异均有显著性 (P <0 .0 1 ) ;激素治疗组血浆SP含量低于正常对照组 (P <0 .0 5 ) ,BALF及肺组织SP含量与对照组比较 ,差异无显著性 (P >0 .0 5 )。哮喘组豚鼠肺组织中SPmRNA相对含量明显高于正常
Objective Substance P (SP) is an important neurotransmitter of non-cholinergic excitatory airway in the airway and is an important neuropeptide that leads to airway neurogenic inflammation in asthma. Whether glucocorticoid can reduce the effect of intrapulmonary SP is unclear, so this paper studies the effect of inhaled glucocorticoid on intrapulmonary SP in asthmatic guinea pig and its molecular mechanism. Methods Thirty guinea pigs were sensitized with ovalbumin and induced asthma. Animals were randomly divided into asthma group and treatment group (15 in each group), and the other 15 guinea pigs were given normal saline as control group. Guinea pigs in the treatment group inhaled beclomethasone propionate atomization solution on the day before induction, on the day of induction and 24 hrs after induction. The asthmatic group and the treatment group were harvested at 24 hours after the induction of asthma, and the control group were drawn at the same time. Bronchoalveolar lavage fluid (BALF), plasma and lung tissue samples were collected respectively. SP protein content was detected by radioimmunoassay. The relative content of SP mRNA in lung tissue was detected by reverse transcription polymerase chain reaction. Results The levels of SP in plasma, BALF and lung tissue of asthmatic group were significantly higher than those of the normal control group [(1 2 2 ± 4 6) pg / mL, (90 ± 39) pg / mL and (78 ± 15) pg / (84 ± 33) pg / ml, (32 ± 21) pg / ml, (42 ± 1 2) pg / ml] and hormone treatment group 7 ± 20) pg / mL and (40 ± 1 3) pg / g protein, respectively). The plasma SP content in hormone therapy group was lower than that in normal control group (P <0.01) <0. 05). There was no significant difference in SP content between BALF and lung tissue in the control group (P> 0.05). The relative content of SP mRNA in asthmatic guinea pigs was significantly higher than normal