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目的:本实验应用体外细胞培养方法研究人参多糖对低温应激大鼠卵巢黄体与颗粒细胞孕酮与cAMP含量的影响,为研究人参多糖提高机体耐寒能力提供科学实验依据。方法:分离培养Wistar雌性大鼠卵巢黄体与颗粒细胞,设37℃对照组、与0℃、-5℃、-10℃、-15℃、-20℃、-25℃低温应激实验组,采用放免分析方法测定孕酮与cAMP生成含量。结果:黄体细胞实验组与对照组基础孕酮为(500.774±56.85)与(837.33±88.23),人参多糖组与对照组为(605.91±36.64)与(500.77±56.85),颗粒细胞实验组与对照组基础孕酮为(128.26±25.46)与(157.74±49.85),人参多糖实验组与对照组为(177.87±52.12)与(128.26±25.46),黄体细胞实验组与对照组cAMP(19.97±11.09)与(14.62±10.40),人参多糖实验组与对照组为(23.64±12.16)与(19.44±15.52),颗粒细胞实验组与对照组基础cAMP为(96.51±10.84)与(89.12±14.04),人参多糖实验组与对照组为(126.31±13.46)与(156.06±16.14)。组间比较,P<0.05。结论:暴寒冷冻应激使大鼠卵巢黄体与颗粒细胞基础孕酮含量与cAMP生成降低,人参多糖则具有上调作用,0～100ng区间呈剂量依赖关系。
OBJECTIVE: To study the effects of ginseng polysaccharides on the levels of progesterone and cAMP in luteal corpus luteum and granulosa cells in vitro, and to provide scientific experimental basis for studying the ability of ginseng polysaccharides to improve the cold resistance of the body. METHODS: Ovarian corpus luteum and granulosa cells were isolated and cultured in Wistar female rats. The control group was set at 37°C, and 0°C, -5°C, -10°C, -15°C, -20°C, -25°C low temperature stress test groups were used. Radioimmunoassay was used to determine progesterone and cAMP production. RESULTS: The progesterone levels in the luteal cell test group and control group were (500.774±56.85) and (837.33±88.23). The ginseng polysaccharide group and the control group were (605.91±36.64) and (500.77±56.85). Granule cell test group and control group The basic progesterone levels in the group were (128.26±25.46) and (157.74±49.85). The ginseng polysaccharides in the experimental and control groups were (177.87±52.12) and (128.26±25.46), and the cAMP in the luteinized and experimental groups (19.97±11.09). With (14.62±10.40), ginseng polysaccharides in the experimental and control groups were (23.64±12.16) and (19.44±15.52). The granulosa cells in the experimental group and the control group were (96.51±10.84) and (89.12±14.04). The polysaccharide experimental group and control group were (126.31±13.46) and (156.06±16.14). Between groups, P<0.05. CONCLUSION: The chilling stress caused a decrease in basal progesterone content and cAMP production in rat corpus luteum and granulosa cells, while ginseng polysaccharides have an up-regulation effect. The 0-100 ng interval is dose-dependent.