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利用我国培育的高强纤维种质系 72 35为材料 ,开展了棉花高强纤维基因或 QTL微卫星标记的筛选。通过2 11对 SSR引物的筛选 ,鉴定出与高强纤维 QTL连锁的 SSR标记 3个 :NAU / SSR/ fs11 30 、NAU / SSR/ fs2 1 90 、NAU /SSR/ fs32 2 0 。 NAU / SSR/ fs11 30 、NAU / SSR/ fs2 1 90 两个标记紧密连锁 ,重组率为 2 .3c M,标记的 QTL占 (72 35× TM- 1)F2 分离群体总遗传变异的 30 .9%。此外 ,这一标记的 QTL在不同年份不同环境表现稳定 ,这是鉴定出的一个控制棉花高强纤维表现的主效位点。NAU/ SSR/ fs32 2 0 与另一个高强纤维 QTL 连锁 ,但遗传效应值小 ,不稳定。单体测验表明 ,NAU/ SSR/ fs11 30 、NAU/ SSR/ fs2 1 90 标记的 QTL 位于第 10染色体上 ,而 NAU/ SSR/ fs32 2 0 标记的 QTL 位于第 5染色体上
Using the high-strength fiber germplasm 72 35 in our country, we carried out the screening of cotton high-fiber genes or QTL microsatellite markers. Three SSR markers linked to the QTLs of high-fiber were identified by 2 11 SSR primers: NAU / SSR / fs11 30, NAU / SSR / fs2 1 90, NAU / SSR / fs32 2 0. The marker QTL accounted for 30.9 of the total genetic variation of F2 segregating population with NAU / SSR / fs11 30 and NAU / SSR / fs2 1 90, with the recombination rate of 2.3cM and the marker QTL of (72 35 × TM-1) %. In addition, the QTL for this marker was stable in different years and in different environments, which was identified as a major site for controlling the performance of cotton high-strength fibers. NAU / SSR / fs32 2 0 is linked to QTL of another high-fiber, but the genetic effect is small and unstable. Monomer tests showed that the QTLs for NAU / SSR / fs11 30 and NAU / SSR / fs2 1 90 were located on chromosome 10 and the QTLs for NAU / SSR / fs32 2 0 were located on chromosome 5