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目的观察通脉降糖胶囊对糖尿病性大鼠周围神经病变的影响。方法 40只Wistar大鼠按随机数字表法分为通脉降糖胶囊组、甲钴胺组、模型组和正常组,每组10只。除正常组外,各组采用链脲佐菌素(streptozotocin,STZ)腹腔注射法制备糖尿病性大鼠周围神经病变模型,造模过程中模型组大鼠死亡1只。成模后第8周各组给药,通脉降糖胶囊组按10 m L/kg给予通脉降糖胶囊(0.23g生药/m L)灌胃,甲钴胺组按10 m L/kg给予甲钴胺与生理盐水的混悬液(0.05 mg/m L)灌胃,至第12周末。同时模型组和正常组给予等量的蒸馏水灌胃。检测各组大鼠周围神经传导速度;进行足迹步态分析;免疫组化染色观察表皮内神经纤维变化;HE染色法观察胫神经组织病理学改变。结果 (1)与正常组比较,模型组大鼠神经传导速度减慢,足迹步态参数足印长(print length,PL)、足中间三指宽(intermediary toe spread,ITS)和足印宽(toe spread,TS)值增加,差异均有统计学意义(P<0.01)。与模型组比较,通脉降糖胶囊组和甲钴胺组神经传导速度增快,PL、ITS值降低,差异均有统计学意义(P<0.01),且通脉胶囊组神经传导速度优于甲钴胺组(P<0.05)。(2)免疫组化染色显示:模型组表皮内神经纤维染色模糊,稀疏零散,不易辨认,局部无神经纤维显示;甲钴胺组神经纤维走形不规则,数量减少,染色较浅,局部无神经纤维显示;通脉降糖胶囊组神经纤维走形不规则,数量减少,断断续续,稀疏零散。(3)HE染色显示:模型组大鼠胫神经组织严重水肿,髓鞘缺失,难以辨认,局部有空泡变性,轴索水肿,部分轴索分离、变性;甲钴胺组胫神经水肿,髓鞘肿胀,不易辨认,轴索局部分离;通脉降糖胶囊组胫神经水肿,髓鞘模糊,轴索肿胀。结论通脉降糖胶囊可以改善糖尿病性大鼠的周围神经病变。
Objective To observe the effects of Tongmai Jiangtang capsule on peripheral neuropathy in diabetic rats. Methods 40 Wistar rats were randomly divided into Tongmai Jiangtang capsule group, mecobalamin group, model group and normal group, with 10 in each group. In addition to the normal group, diabetic rats peripheral neuropathy model was established by intraperitoneal injection of streptozotocin (STZ) in each group. One rat died in the model group during the modeling. The rats in the Tongmai Jiangtang Capsule group were administered with Tongmai Jiangtang Capsule (0.23g crude drug / m L) at the dose of 10 m L / kg and the mice in the mecobalamine group were treated with 10 m L / kg A suspension of methylcobalamin and saline (0.05 mg / mL) was given orally until the end of the 12th week. At the same time model group and normal group given the same amount of distilled water gavage. Peripheral nerve conduction velocity was measured in each group. Footprints gait analysis was performed. The changes of nerve fibers in the epidermis were observed by immunohistochemical staining. The histopathological changes of the tibial nerve were observed by HE staining. Results (1) Compared with the normal group, the nerve conduction velocity of the model group was slowed down, the footprints of the footprints of the footprints were longer than those of the normal group (print length, PL), ITS and footprints Toe spread, TS) increased, the differences were statistically significant (P <0.01). Compared with the model group, the nerve conduction velocity of Tongmai Jiangtang capsule group and mecobalamin group increased rapidly, PL and ITS values decreased, the differences were statistically significant (P <0.01), and the nerve conduction velocity of Tongmai capsule group was superior to Mecobalamin group (P <0.05). (2) Immunohistochemical staining showed that the nerve fibers in the epidermis of the model group were stained fuzzy, sparse and scattered, not easily identifiable, and showed no nerve fibers in the local area. The melatonin group had irregularly shaped and reduced numbers of nerve fibers, Nerve fibers showed that Tongmai Hypoglycemic Capsule group had irregularly shaped nerve fibers and decreased numbers, intermittent and sparse. (3) Hematoxylin-eosin staining showed that the tibial nerve tissue in model group had severe edema and myelin sheath loss, which was difficult to identify. Local vacuolar degeneration, axonal edema, partial axonal separation and degeneration; Sheath swelling, not easy to identify, axonal local separation; Tongmai Jiangtang capsule group tibial nerve edema, myelin fuzzy, axial swelling. Conclusion Tongmai Jiangtang capsule can improve diabetic peripheral neuropathy in rats.