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目的 探讨黄芪当归的治疗机理,并试图寻找黄芪的有效作用组分。方法 采用核转录活性测定(nuclear run-on assay)和分子杂交的方法观察了肾病综合征大鼠肝脏白蛋白基因的转录活性和白蛋白mRNA的表达水平。结果 肾病组转录活性高于对照组,而黄芪当归治疗组又高于肾病组。白蛋白mRNA的表达水平的变化趋势与之相吻合。用单味黄芪或黄芪的三个单组分治疗的各组大鼠白蛋白基因转录活性及mRNA表达水平与肾病组比无显著差异,但在血浆白蛋白水平有显著差异,且黄芪多糖Ⅱ的作用效果最强。结论 黄芪当归合剂能促进白蛋白基因转录活性,而单味黄芪和黄芪的三个组分改善肾病鼠蛋白质代谢紊乱不通过该机制。
Objective To investigate the therapeutic mechanism of Angelica sinensis Angelica and to search for effective components of Radix Astragali. Methods The transcriptional activity of liver albumin gene and albumin mRNA expression in rats with nephrotic syndrome were observed by nuclear run-on assay and molecular hybridization. Results The transcriptional activity of nephropathy group was higher than that of the control group, while the treatment group of Astragalus membranaceus was higher than that of nephropathy group. The change in the expression level of albumin mRNA coincided with it. The albumin gene transcriptional activity and mRNA expression levels in each group of rats treated with three monocomponents of Astragalus membranaceus or Astragalus membranaceus were not significantly different from those in nephropathy group, but there was a significant difference in plasma albumin level, and Astragalus polysaccharide II The most effective effect. Conclusion Astragalus Angelica mixture can promote the transcriptional activity of albumin gene, while the three components of Astragalus membranaceus and Astragalus membranaceus can improve protein metabolism disorder of nephropathy rats not through this mechanism.