论文部分内容阅读
用ＰＣＲ产物进行克隆已广泛用于分子生物学的各个领域。ＰＣＲ产物的克隆主要通过两个途径：其一，在引物的３’末端加上限制性内切酶的识别序列，使扩增片段产生粘性末端，再连接到有相应末端的载体中去。但是，由于许多限制酶对处在ＤＮＡ片段末端的识别序列的作用效率远较?
Cloning with PCR products has been widely used in various fields of molecular biology. The cloning of PCR products mainly through two ways: First, in the 3 ’end of the primer with restriction endonuclease recognition sequence, the amplified fragments produce sticky ends, and then connected to the corresponding ends of the vector to go. However, since many restriction enzymes are far more efficient at recognizing sequences at the ends of DNA fragments?