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目的研究荷叶中的化学成分。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及PHPLC法进行分离纯化,并通过理化性质、光谱分析方法及与文献对比,鉴定化合物的化学结构。结果从荷叶体积分数为70%的乙醇提取物中分离鉴定了8个化合物,其中有4个木脂素类:(+)-松脂醇[(+)-pinoresinol,1]、(+)-表松脂醇[(+)-epipinoresinol,2]、sylvatesmin(3)、(+)-异落叶松树脂醇[(+)-isolariciresinol,4];4个降倍半萜类:(3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-me-gastigmen-9-one(5)、4,5-dihydroblumenol A(6)、(E)-3-oxo-retro-α-ionol(7)、(3S,5R,6R,7E,9S)-megastigman-7-ene-3,5,6,9-tetraol(8)。结论化合物1-8均为首次从莲属植物中分离得到。
Objective To study the chemical composition of lotus leaf. Methods The compounds were isolated by normal phase silica gel, reversed phase ODS, Sephadex LH-20 column chromatography and PHPLC method. The chemical structures of the compounds were identified by physical and chemical properties, spectral analysis and comparison with the literature. Results Eight compounds were isolated and identified from ethanol extracts with a volume fraction of lotus leaf (70%), of which four were lignans: (+) - pinoresinol, 4-Sesquiterpene: (3S, 5R, 5-Methylthio-2-methylpropanoid) (5), 4,5-dihydroblumenol A (6), (E) -3-oxo-retro-a- ionol (7), (3S, 5R, 6R, 7E, 9S) -megastigman-7-ene-3,5,6,9-tetraol (8). Conclusion Compounds 1-8 were isolated from genus Lotus for the first time.