p16INK4a/CDKN2A基因家族及其相关因子所组成的庞大调控体系是细胞周期最关键的调节通路之一 ,与其他正负调控因子协调合作 ,决定着细胞周期的正常运转和细胞增殖、分化、老化及凋亡 ,并与多种人类肿瘤的发生、发展、治疗与预后密切相关。本文就多肿瘤抑制基因p16INK4a/CDKN2A发现以来 ,国内外关于其基因结构、功能、失活机制及其与多种人类肿瘤的相关性研究作一综述 ,并展望其在肿瘤生物治疗中的应用前景。 The p16INK4a/CDKN2A gene family and its related regulatory factors constitute one of the most critical regulatory pathways in the cell cycle, and coordinate with other positive and negative regulators to determine the normal operation of the cell cycle and cell proliferation, differentiation, and aging. Apoptosis is closely related to the occurrence, development, treatment and prognosis of various human tumors. In this paper, we review the research on the structure, function, inactivation mechanism and its correlation with a variety of human tumors at home and abroad since the discovery of the multiple tumor suppressor gene p16INK4a/CDKN2A, and look forward to its application prospects in tumor biotherapy. .