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目的:建立体外髁突器官培养系统,明确体外培养髁突软骨的生物学特性,为髁突软骨体外实验提供基础。方法:选用新生SD大鼠的髁突软骨作为器官培养材料,采用格栅式器官培养法,建立体外髁突软骨器官培养模型。分别在取材培养后1~6 d收集样本,制备组织切片并观察。结果:培养早期(4 d以内),髁突软骨层次分明,细胞结构完整,形态良好。培养时间延长至5 d,软骨表层局部出现空腔。结论:体外器官培养的髁突软骨在4d内能维持良好的形态和生长状态,具有生物活性,可以应用于髁突软骨的相关体外实验研究。
OBJECTIVE: To establish an in vitro condylar organ culture system to identify the biological characteristics of condylar cartilage in vitro and provide the basis for in vitro experimental condylar cartilage. Methods: The condylar cartilage of neonatal SD rats was selected as organ culture material. The organ culture model of condylar cartilage in vitro was established by the method of organ culture. Samples were harvested 1 to 6 days after the culture, and tissue sections were prepared and observed. Results: In the early stage of training (within 4 days), the condylar cartilage was well-differentiated and the cell structure was intact with good morphology. The culture time was extended to 5 days, the local appearance of the cartilage cavity. CONCLUSION: Condylar cartilage cultivated in vitro can maintain good morphology and growth status within 4 days and has biological activity. It can be used in the related in vitro experimental study of condylar cartilage.