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目的研究腹腔注射、灌胃、皮肤染毒丙烯酰胺(AA)对小鼠早期精细胞微核发生率的影响,以反映相应的遗传毒性。方法将24只清洁级健康雄性昆明小鼠随机分为4组,分别为空白对照组、腹腔注射染毒组、灌胃染毒组和皮肤染毒组,每组6只。染毒剂量为25 mg/kg,每天1次,连续染毒5 d。首次染毒后第19天称体重和睾丸重量,计算睾丸系数。采用早期精细胞微核实验测定各组小鼠早期精细胞微核的发生率。结果染毒后腹腔注射染毒组、灌胃染毒组和皮肤染毒组小鼠体重均低于空白对照组,差异有统计学意义(P<0.05或P<0.01);与空白对照组比较,腹腔注射染毒组、灌胃染毒组和皮肤染毒组小鼠的睾丸重量均较低,差异有统计学意义(P<0.05);与空白对照组比较,腹腔注射染毒组和皮肤染毒组小鼠早期精细胞微核发生率较高,但差异无统计学意义(P>0.05),灌胃染毒组小鼠早期精细胞微核发生率高,差异有统计学意义(P<0.05)。结论 AA可致雄性小鼠早期精细胞微核发生率增高,且以灌胃染毒方式产生的毒性作用最为敏感。
Objective To study the effects of intraperitoneal injection, intragastric administration, and dermal administration of acrylamide on the incidence of early micronuclei in mice to reflect the corresponding genotoxicity. Methods Twenty-four healthy male Kunming mice of clean level were randomly divided into 4 groups: blank control group, intraperitoneal injection, intragastric and dermal exposure groups, 6 in each. The dose was 25 mg / kg once daily for 5 days. On the 19th day after the first exposure, weighed the body weight and testicular weight and calculated the testicular coefficient. Early spermicidal micronucleus test was used to determine the incidence of spermicellular micronuclei in each group. Results Compared with the blank control group, the mice in the intraperitoneal injection exposure group, the intragastric administration group and the skin exposure group had lower body weight than the blank control group (P <0.05 or P <0.01) (P <0.05). Compared with the blank control group, the weight of testis in mice in intraperitoneal injection and skin exposure groups was lower than that in control group The incidence of spermicidal micronuclei in the early-stage mice was higher than that in the untreated mice (P> 0.05), and the incidence of micronuclei in spermatogenic cells was higher <0.05). Conclusion The incidence of micronuclei in spermatogenic cells in AA-induced male mice increased at the early stage, and the toxicity induced by gavage was the most sensitive.