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目的:研究复方当归汤含药肠吸收液舒张血管平滑肌的作用。方法:取大鼠小肠制成翻转囊,置于复方当归汤粗提液中温孵2 h,取肠囊内K-R液即得复方当归汤含药肠吸收液。采用大鼠离体胸主动脉环灌流模型,观察累积浓度含药肠吸收液对基础状态、苯肾上腺素(PE)预收缩、氯化钾预收缩血管环的作用,并与硝苯地平比较。结果:复方当归汤含药肠吸收液对基础状态或氯化钾预收缩的血管环无明显影响;当含药肠吸收液中指标成分阿魏酸浓度累积达2.48×10-3,4.96×10-3,9.92×10-3g.L-1时,可剂量依赖性抑制PE预收缩的血管环收缩(P<0.05)。硝苯地平对基础状态预收缩的血管环无明显影响;当浓度累积达8.66×10-4,17.32×10-4,34.65×10-4mmol.L-1时,可剂量依赖性抑制PE或氯化钾预收缩的血管(P<0.05,或P<0.01)。结论:复方当归汤含药肠吸收液对完整内皮的PE预处理的血管有舒张作用,含药肠吸收液可用于中药复方离体药效学研究。
Objective: To study the effect of compound Danggui decoction-containing intestinal fluid on vasodilation of vascular smooth muscle. Methods: The small intestine of rats was made into flip-flops and placed in the crude extract of compound Angelica decoction for 2 h. The K-R liquid in the intestine was obtained for the compound danggui decoction-containing intestinal absorption solution. The isolated rat thoracic aorta was used to observe the effect of cumulative concentration of medicated intestinal absorption solution on the basal state, the precontraction of phenylephrine (PE) and the precontracted vascular rings of potassium chloride, and compared with nifedipine. Results: The compound Angelica Decoction-containing intestinal absorption solution had no significant effect on the vascular rings in basal state or precontracted potassium chloride. When the content of ferulic acid in the medicated intestinal absorption solution reached 2.48 × 10-3 and 4.96 × 10 -3,9.92 × 10-3g.L-1, it can inhibit the contractile contraction of PE in a dose-dependent manner (P <0.05). Nifedipine had no significant effect on the precontracted vascular rings in the basal state; when the concentration reached 8.66 × 10-4, 17.32 × 10-4 and 34.65 × 10-4mmol.L-1, the dose-dependent inhibition of PE or chlorine Potassium precontracted blood vessels (P <0.05, or P <0.01). Conclusion: The compound Angelica Decoction-containing intestinal absorption solution can relax the vascular endothelial cells pretreated with intact endothelium, and the medicated intestinal absorption solution can be used for pharmacokinetic studies in vitro.