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研究库普弗细胞（ＫＣ）是否参与小鼠免疫性肝损伤．方法：给小鼠静脉注射卡介苗（ＢＣＧ）５×１０７活菌后再静脉注射脂多糖（ＬＰＳ）７５μｇ以诱导免疫性肝损伤．以维生素Ａ激活ＫＣ和以印度墨汁或硅砂封闭ＫＣ后，测定血浆一氧化氮（ＮＯ），谷丙转氨酶（ＡｌａＡＴ），谷草转氨酶（ＡｓｐＡＴ）的变化并检查肝组织的病理改变．结果：注射ＢＣＧ后，再注射ＬＰＳ７５μｇ，可导致小鼠血浆ＮＯ，ＡｌａＡＴ，ＡｓｐＡＴ剧烈升高及严重的肝损伤．以维生素Ａ激活ＫＣ后，肝损伤更为严重，而以印度墨汁或硅砂封闭ＫＣ后，肝损伤则显著减轻．结论：ＢＣＧ＋ＬＰＳ诱导的小鼠肝损伤与ＫＣ的功能关系密切，来源于ＫＣ的ＮＯ在ＢＣＧ＋ＬＰＳ诱导的肝损伤中起重要作用．
To investigate whether Kupffer cells (KCs) are involved in immunological liver injury in mice. Methods: The mice were injected intraperitoneally with 5 × 10 7 BCG of Bacillus Calmette-Guérin (BCG) and then intravenously injected with lipopolysaccharide (LPS) at 75 μg to induce immune liver injury. After activating KC with vitamin A and blocking KC with Indian ink or silica sand, the changes of plasma nitric oxide (NO), alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT) were measured and pathological changes in liver tissues were examined. Results: After injection of BCG, injection of 7.5 μg of LPS resulted in the dramatic increase of plasma NO, AlaAT, AspAT and severe liver injury in mice. Liver injury was more severe with vitamin A-activated KC, whereas liver injury was significantly reduced after KC was closed with Indian ink or silica sand. CONCLUSION: Liver injury induced by BCG + LPS is closely related to the function of KC. NO derived from KC plays an important role in liver injury induced by BCG + LPS.