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目的建立免疫亲和柱净化-光化学衍生液相色谱法,测定植物油中黄曲霉毒素B1(AFB1)的含量。方法用水+甲醇+乙腈/提取样品中AFB1,经免疫亲和柱净化、液相色谱分离、光化学柱后衍生,荧光检测器测定。结果AFB1的检出限0.18!g/kg,回收率86.3%~103.0%,相对标准偏差(RSD)3.18%~4.12%。结论该方法符合植物油中AFB1的检测要求,操作简便快速、准确、重现性好。
Objective To establish an immunoaffinity column purification-photochemical derivatization liquid chromatography (HPLC) to determine the content of aflatoxin B1 (AFB1) in vegetable oil. Methods AFB1 was extracted by water + methanol + acetonitrile / acetonitrile, purified by immunoaffinity column, separated by liquid chromatography and determined by fluorescence detector. Results The detection limit of AFB1 was 0.18 μg / kg, the recoveries were 86.3% -103.0% and the relative standard deviations (RSDs) were 3.18% -4.12%. Conclusion The method meets the detection requirements of AFB1 in vegetable oil. The method is simple, rapid, accurate and reproducible.