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为了解柯萨奇病毒B1型(Coxsackievirus B1,CV-B1)山东地方株的分子流行病学特征,本研究对1994年至2015年山东省急性弛缓性麻痹(Acute flaccid paralysis,AFP)监测系统、环境污水监测和无菌性脑膜炎病例标本中分离到的CV-B1病毒进行了VP1序列测定、系统发生学分析和同源性分析。共分离到CV-B1病毒53株,其中AFP监测、污水和及脑炎标本各分离到41株、4株和8株。基于VP1完整编码区序列的系统发生学分析显示CVB1山东株与国内其他分离株属于一个大的分支,该分支内无国外分离株,国外分离株构成了其他两个分支。山东株之间的VP1核苷酸同源性为84.4%~100.0%,与其他国家分离株的同源性为77.9%~85.0%。研究结果表明,中国CV-B1分离株与国外株相比有较大的遗传差异,需要加强相关手足口病病毒学监测,关注不同传播链的新的基因亚型的肠道病毒的输入。
In order to understand the molecular epidemiological characteristics of Coxsackievirus B1 (CV-B1) Shandong local strains, this study analyzed the surveillance system of acute flaccid paralysis (AFP) in Shandong Province from 1994 to 2015, VP1 sequence analysis, phylogenetic analysis and homology analysis of CV-B1 virus isolated from environmental sewage monitoring and aseptic meningitis cases. A total of 53 strains of CV-B1 virus were isolated, of which 41 strains, 4 strains and 8 strains were isolated from AFP surveillance, sewage and encephalitis samples. Phylogenetic analysis based on the complete coding region of VP1 showed that Shandong strain CVB1 belonged to a large branch with other domestic isolates. There were no foreign isolates in this branch, and the other isolates constituted two other branches. The nucleotide homology of VP1 between Shandong strains ranged from 84.4% to 100.0%, and the homologies with other isolates ranged from 77.9% to 85.0%. The results showed that the CV-B1 isolates in China had a large genetic difference compared with foreign strains. Virulence monitoring of hand-foot-mouth disease should be strengthened, and the input of new gene subtypes of enteroviruses with different transmission chains should be emphasized.