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在制备完成高通量稻瘟病菌(Magnaphorthe grisea)cDNA阵列的基础上,平行检测孢子受疏水性表面诱导0、2、8、20和30h的基因表达谱。结果显示,在附着胞形成的不同阶段,均存在大量的差异表达基因,显示出这一过程中基因表达网络的复杂性。其中,附着胞在成熟阶段的差异表达基因数要明显高于其诱导和形成阶段,表现在基因表达谱20和30h时间点后发生剧烈变化。这种动态的基因表达谱的分析可以为稻瘟病菌致病的分子机理研究提供有意义的信息。
On the basis of the completed Magnaphorthe grisea cDNA array, the gene expression profiles of spores at 0, 2, 8, 20 and 30 h were induced by hydrophobic surface. The results showed that there are a large number of differentially expressed genes in different stages of appressorium formation, indicating the complexity of the gene expression network in this process. Among them, the number of differentially expressed genes in adherent cells at the mature stage was significantly higher than that at the induction and the formation stage, showing dramatic changes after 20 and 30 h of gene expression profiling. The analysis of this dynamic gene expression profile can provide meaningful information on the molecular mechanism of pathogenicity of Magnaporthe grisea.