论文部分内容阅读
目的探讨miR-155对人大肠癌细胞侵袭能力的影响。方法大肠癌Lovo细胞分为3组:脂质体介导反义miR-155(AS-miR-155)组、无义寡脱氧核苷酸(ODN)组和对照组。测定荧光素酶活性验证3组细胞中miR-155的表达,采用Matrigel基质生长试验检测细胞生长情况,以Transwe Ⅱ方法检测细胞的侵袭力,结果与对照组和无义ODN组比较,转染AS-miR-155组Lovo细胞miR-2l表达水平降低;Matrigel基质生长试验显示,转染AS-miR-155组Lovo细胞体外培养克隆平均直径较小,Transwe Ⅱ细胞侵袭试验显示转染AS-miR-155组穿膜细胞数较少。结论 miR-2l高表达可促进Lovo大肠癌细胞侵袭生长,提示miR-155可以作为基因治疗大肠癌的候选靶点。
Objective To investigate the effect of miR-155 on the invasion of human colorectal cancer cells. Methods Lovo cells from colorectal cancer were divided into three groups: liposome-mediated antisense miR-155 (AS-miR-155) group, nonsense oligodeoxynucleotide (ODN) group and control group. The luciferase activity was measured to verify the expression of miR-155 in three groups of cells. Matrigel growth assay was used to detect cell growth. Transwell II was used to detect the invasiveness of the cells. The results were compared with the control group and the non-sense ODN group. - miR-155 group decreased the expression of miR-2l in Lovo cells; Matrigel stromal growth assay showed that the average diameter of clones transfected with Lovo cells transfected with AS-miR-155 in vitro was smaller, Transwe II cell invasion assay showed transfection of AS-miR- The number of membrane cells in the 155 group was less. Conclusion The high expression of miR-2l can promote the invasion and growth of Lovo colorectal cancer cells, suggesting that miR-155 can be used as a candidate target for gene therapy for colorectal cancer.