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目的 探讨纤状蛇纹石石棉致肺泡巨噬细胞受损的机制。方法 采用体外细胞培养技术 ,以兔肺泡巨噬细胞死亡率、乳酸脱氢酶 (LDH)、超氧化物歧化酶 (SOD)活力及丙二醛 (MDA)含量变化来表示不同剂量、不同培养时间的纤状蛇纹石石棉对巨噬细胞的毒性。结果 纤状蛇纹石石棉对肺泡巨噬细胞的毒性存在着剂量 效应关系、时间 效应关系 ;12 5 μg/ml培养 18h后 ,细胞死亡率及LDH增加 ,5 0 0 μg/ml培养 5h后 ,细胞死亡率、LDH、MDA均明显增加 ,而SOD活力明显降低。随粉尘剂量的增加及培养时间的延长 ,细胞毒性也增加。结论 纤状蛇纹石石棉对肺泡巨噬细胞的毒性可能与其表面活性基团有关
Objective To investigate the mechanism of impaired alveolar macrophages induced by fibrous serpentine asbestos. Methods The in vitro cell culture technique was used to express the changes of the death rate of alveolar macrophages, the activity of lactate dehydrogenase (LDH), superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in rabbits with different doses, different culture time The banded serpentine asbestos is toxic to macrophages. Results There was a dose-response and time-effect relationship between fiber-like serpentine asbestos and alveolar macrophages. The cell death rate and LDH increased after 125 μg / ml of culture for 18 hours. After cultured at 500 μg / ml for 5 hours, Cell death rate, LDH, MDA were significantly increased, while SOD activity was significantly reduced. With increasing dust dose and incubation time, cytotoxicity also increased. Conclusion The nodular serpentine asbestos may be related to its surface active groups on alveolar macrophages