PKC在细胞生长、分化、凋亡和信号转导调节中具有重要作用.通过激光扫描共聚焦显微镜证实:在胃癌BGC-823细胞中,一部分PKCα定位于线粒体,一部分定位在胞浆,细胞经TPA处理后,位于线粒体和胞浆的PKCα向细胞核转运;Western blot检测则发现PKCα蛋白表达水平在TPA处理前后没有发生变化.此外,应用凋亡诱导剂和特异性PKC抑制剂的实验结果进一步证实:胃癌细胞内PKCα由线粒体和胞浆向细胞核转运与细胞凋亡的诱导密切相关.提示PKCα在细胞内的定向转运可能是与细胞凋亡过程相关联的重要事件之一. PKC plays an important role in the regulation of cell growth, differentiation, apoptosis and signal transduction.It is confirmed by laser scanning confocal microscopy that in the gastric cancer BGC-823 cells, part of PKCα is located in the mitochondria, partly in the cytoplasm, After treatment, PKCα located in mitochondria and cytoplasm were transported to the nucleus, Western blot analysis showed that PKCα protein expression level did not change before and after TPA treatment.In addition, using the apoptosis inducer and specific PKC inhibitors further confirmed that: PKCα in gastric cancer cells is closely related to the induction of apoptosis by mitochondria and cytoplasm to the nucleus, suggesting that the directional transport of PKCα in the cells may be one of the important events associated with the apoptosis process.