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目的:探讨新生儿指尖皮肤及皮下组织中树突状细胞的形态、数量和分布情况。方法:5例尸检新生儿无名指指皮组织样本,行免疫组织化学染色(CD1a、CD209和MHCⅡ标记),光镜观察。结果:新生儿指皮组织中,阳性细胞呈棕褐色,大小不等、形态多样,有数量不等的突起,呈散在或灶带状分布。CD1a标记的细胞阳性率为(23±11.9)%,多位于表皮乳头周围,血管外膜以及真皮疏松结缔组织中;CD209标记的细胞阳性率为(30±7.7)%,多位于真皮层内,血管神经分叉处,血管外膜,环层小体被囊结缔组织中;MHCⅡ的标记的细胞阳性率(8±1.9)%,多位于血管外膜及周围疏松结缔组织中。结论:稳态下,新生儿皮肤组织中存在多种分化发育程度不同的DCs(且以不成熟DCs为主),是皮肤免疫微环境的重要组成部分。
Objective: To investigate the morphology, quantity and distribution of dendritic cells in the skin and subcutaneous tissue of newborn infants. Methods: The autopsy specimens of 5 newborn infants were examined by immunohistochemical staining (CD1a, CD209 and MHC Ⅱ) and observed with light microscope. Results: Neonatal finger tissue, positive cells were brown, ranging in size, shape and diversity, there are a number of protrusions, scattered or zonal distribution. The positive rate of CD1a-labeled cells was (23 ± 11.9)%, mostly located in the epidermal papilla, adventitia and loose connective tissue. The positive rate of CD209-labeled cells was (30 ± 7.7)%, Vascular nerve bifurcation, vascular adventitia, annular bodies were cystic connective tissue; MHC Ⅱ labeled cells positive rate (8 ± 1.9)%, mostly in the adventitia and surrounding loose connective tissue. Conclusion: There are many DCs with different degrees of differentiation and development (mainly immature DCs) in the skin of newborns at steady state, which is an important part of immune immune microenvironment.