论文部分内容阅读
目的:探讨人脐血间充质干细胞体外培养过程中的终末分化方向。方法:采用密度梯度离心法从人脐血中分离培养间充质干细胞,流式细胞术(FCM)检测细胞表面抗原标记CD29、CD44、CD105、CD34、CD106和HLA-DR;观察人脐血间充质干细胞在体外培养过程中的增殖情况和形态变化,应用RT-PCR和免疫细胞化学方法对P2和P10代人脐血间充质干细胞进行神经元特异性烯醇化酶(NSE)和神经丝蛋白(NF)的表达情况进行检测。结果:RT-PCR分析证实P10代人脐血间充质干细胞有NSE、NF的mRNA表达,免疫细胞化学检测显示P10代细胞能表达NSE和NF,而P2代人脐血间充质干细胞经RT-PCR和免疫细胞化学检测均无NSE、NF表达。结论:人脐血间充质干细胞在体外培养过程中能终末分化为神经元样细胞。
Objective: To investigate the direction of terminal differentiation of human umbilical cord blood mesenchymal stem cells cultured in vitro. Methods: Mesenchymal stem cells (MSCs) were isolated and cultured from human umbilical cord blood by density gradient centrifugation. The surface markers CD29, CD44, CD105, CD34, CD106 and HLA-DR were detected by flow cytometry (FCM) Proliferation and morphological changes of MSCs during in vitro culture were evaluated by RT-PCR and immunocytochemistry. The results showed that neuron-specific enolase (NSE) and neurofilament of P2 and P10 human umbilical cord blood derived mesenchymal stem cells Protein (NF) expression was detected. Results: The mRNA expression of NSE and NF in P10-derived human umbilical cord blood derived mesenchymal stem cells was confirmed by RT-PCR analysis. Immunocytochemistry showed that NSE and NF were expressed in P10 passaged cells, No expression of NSE and NF was detected by PCR and immunocytochemistry. Conclusion: Human umbilical cord blood mesenchymal stem cells can differentiate into neuron-like cells in vitro during culture.